Effect of selective gut decontamination on peritoneal fibrinolytic activity in rats
Özer İlkgül1, Yamaç Erhan1, Hasan Aydede1, Ahmet Var2, Ece Onur2
1Celal Bayar Üniversitesi Genel Cerrahi AD, MANİSA
2Celal Bayar Üniversitesi Biokimya AD, MANİSA
Abstract
Purpose: Our goal with this experiment was to determine the influence of selective decontamination of the digestive tract (SDD) on peritoneal fibrinolytic activity in rats.
Materials and Methods: Animals were divided into five groups. Group 1 was subjected to SDDtreated peritoneal adhesion. Group 2 was subjected only to perioneal adhesion. Group 3 was SDD-treated, whereas Group 4 was labeled as the control group. Group 5 was subjected to simple laparotomy. The rats in Group 1 and Group 3 were given SDD (tobramycin 20 mg/l and polymyxin E 25 mg/l) by gavage twice daily for 3 days preceding surgical operation. Control groups were fed orally with standard chow. After a 72-hour waiting period following the operation, biopsies of undamaged parietal peritoneum were obtained from the fibrinolytic parameters in order to measure tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor type-1 (PAI-1).
Results: Average tPA levels were low in both the sdd-treated group and the sdd+adhesion model group, however no statistical significance was elicited. PAI-1 levels were higher in sddtreated groups than in other groups, but no significantly meaningful difference was obtained.
Conclusion: These results suggest that pretreatment with sdd reduces the peritoneal fibrinolytic activity and might also enhance the peritoneal adhesion formation in rats.
Keywords: Selektif bağırsak dekontaminasyonu, periton, fibrinolizis.