Can heat shock protein 32 be used for the early diagnosis of acute mesenteric ischemia?
Sait Berhuni1, Ersin Öztürk1, Arzu Yılmaztepe Oral2, Pınar Sarkut1, Nevzat Kahveci3, Tuncay Yılmazlar1, Kasım Özlük3, Ömer Yerci4
1Department of General Surgery, Uludağ University School of Medicine, Bursa, Turkey
2Department of Biochemistry, Uludağ University School of Medicine, Bursa, Turkey
3Department of Physiology, Uludağ University School of Medicine, Bursa, Turkey
4Department of Pathology, Uludağ University School of Medicine, Bursa, Turkey
Objective: Acute mesenteric ischemia is a challenging and fatal disease. The aim of this study was to detect the heat shock protein 32 (HSP32) response in intestinal tissue and systemic blood to intestinal ischemia and ischemia/reperfusion to define a tool for the early diagnosis of acute mesenteric ischemia.
Material and Methods: Thirty female Wistar albino rats were equally divided into 3 groups. Group 1 rats underwent simple laparotomy and closure (control). In Group 2 rats, 1-hour intestinal ischemia followed by 5-hour reperfusion was performed, and Group 3 rats were subjected to 6-hour intestinal ischemia. The experiment was repeated with a 24-hour waiting period. At the end of the waiting period, blood was withdrawn from the tail veins of the rats and the rats were sacrificed via cardiac puncture. Re-laparotomy was subsequently performed and intestinal tissue and luminal samples were obtained for biochemical and pathological investigations. The HSP32 levels of intestinal tissues, luminal contents and blood levels were compared among the groups.
Results: At the end of the 24-hour waiting period, the median tissue HSP32 levels were 0.43 (0-6.6) ng/mL for Group 1, 9.51 (2.5-49.9) ng/mL for Group 2 and 43.13 (6.3-121.3) ng/mL for Group 3 (p=0.001). The median blood HSP32 levels were 0.11 (0.1-1.4) ng/mL for Group 1, 0.42 (0.1-0.7) ng/mL for Group 2, and 0.25 (0.1-1.2) ng/mL for Group 3 (p=0.047). The HSP levels in the luminal contents were undetectable.
Conclusion: Both ischemia and ischemia/reperfusion significantly raised intestinal tissue HSP32 levels in comparison with the control group. However, this change was not reflected in the circulating blood or luminal contents.
Keywords: Acute, heat shock protein 32, ischemia/reperfusion, mesenteric ischemia
Ethics committee approval was received for this study from the Uludağ University Ethics Committee for Animal Studies.
Concept - E.Ö.; Design - E.Ö., N.K., K.Ö.; Supervision - T.Y., K.Ö.; Resources - S.B.; Materials - S.B., P.S.; Data Collection and/or Processing - S.B., P.S., A.O.; Analysis and/or Interpretation - E.Ö., A.O., Ö.Y.; Literature Search - P.S., E.Ö.; Writing Manuscript - S.B., E.Ö., P.S., N.K.; Critical Review - A.O., T.Y., K.Ö.
No conflict of interest was declared by the authors.
The authors declared that this study has received no financial support.