THE EFFECT OF LOW DOSES LIPOPOLYSACCHARIDE PRETREATMENT IN THE PREVENTION OF BACTERIAL TRANSLOCATION IN ACUTE NECROTIZING PANCREATITIS

Abstract

This study was aimed to examine whether low dose LPS could affect bacterial translocation (BT) in acute pancreatitis (AP). Rats were divided into three groups, LPS, control and sham groups. AP was induced in LPS and control groups by pressure injection of 5% taurocholate into the biliopancreatic duct (1.5mL/kg). LPS rats received LPS (50 mg/kg, intraperitoneally); control and sham rats received a similar volume of normal saline as placebo 24 hours before the induction of pancreatitis. At 24 hours postoperatively, blood was drawn from periphery and portal ven for culture, serum AST, ALT, LDH, amylase, endotoxin and TNF-alpha (TNF-alpha) determinations Specimens from MLNs, spleen, liver and cecum were harvested for culture. There was no difference in serum AST, ALT, LDH and amylase levels and the mean pancreatic histology score between LPS and control (p>0.05), respectively. All these parameters were significantly higher in the LPS and control groups compared with sham group (p<0.01). Serum TNF-alpha and endotoxin level were higher in control and LPS groups compared with sham group (p<0.01) and were lower in the LPS group compared with control group (p<0.01 ). Two of 7 LPS rats had BT to MLNs, compared with 6 of 7 rats in control. Four of 7 LPS rats had BT to distant sites such as spleen ,liver, and/or blood, compared with 7 of 7 rats in control. Low dose LPS treatment decreases bacterial spread to distant sites increases serum TNF-alpha and endotoxin levels but does not reduce serum amylase, AST, ALT, LDH levels or ameliorate pancreatic damage in rats with AP.