Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions
Erdal Uysal1, Mehmet Dokur2, Türkay Kırdak3, Akif Kurt4, Mehmet Karadağ5
1Department of General Surgery, Sanko University School of Medicine, Gaziantep, Turkey
2Department of Emergency Medicine, Necip Fazıl City Hospital, Kahramanmaraş, Turkey
3Department of General Surgery, Uludağ University School of Medicine, Bursa, Turkey
4Department of Pharmacology, Kahramanmaraş Sütçü İmam University School of Medicine, Kahramanmaraş, Turkey
5Department of Biostatistic and Medical Informatics, İnönü University Health Sciences Institue, Malatya, Turkey
Abstract
Objective: The goal was to examine the efficiency of local implementation of adipose-derived mesenchymal stem cells, which have an anti-inflammatory effect, in preventing the intra-abdominal adhesions in rats.
Material and Methods: Twenty-one Wistar albino rats were randomly divided into 3 groups, 7 rats in each: Group 1 was defined as the control group, Group 2 as the sham group, and Group 3 as the adipose-derived mesenchymal stem cell group. A 6 cm mid-abdomen incision in the all the rats was performed. The cecum serosa and sub-serosa were injured by rubbing with a gauze. No agent was applied intraperitoneally for the rats in Group 1; 1.5 mL saline and 2x106/kg allojenic adipose-derived mesenchymal stem cells in the 1.5 mL saline were injected into peritoneum of rats in Groups 2 and 3, respectively. Laparotomy was performed on the 14th day. Adhesion scores, histopathological examination, E-cadherin expression, and the tissue hydroxyproline level were evaluated.
Results: The general adhesion score and collagen deposition in Group 3 were found to be significantly higher than in Groups 1 and 2 (p=0.003 and p=0.009, respectively). In the inflammatory cell comparison, a significant decrease was found in Group 3 in proportion to Groups 1 and 2 (p=0.001, p=0.005, respectively). The E-cadherin levels were found to be higher in Group 3 (p=0.003).
Conclusion: Severe adhesion was observed in the adipose-derived mesenchymal stem cells group. Collagen intensity and E-Cadherin expression also increased in the adipose-derived mesenchymal stem cells group. The anti-inflammatory effect was also seen in the adipose-derived mesenchymal stem cells group.
Keywords: E-cadherin, rat, hydroxyproline, peritoneal adhesion, stem cells
Cite this paper as: Uysal E, Dokur M, Kırdak T, Kurt A, Karadağ M. Evaluation of the effects of adipose-derived mesenchymal stem cells on intraperitoneal adhesions. Turk J Surg 2018; 10.5152/turkjsurg.2017.3860.
Ethics committee approval was received for this study from the Ethics Committee of Kahramanmaras Sutcu Imam University (2016/01-02).
Not required in this study.
Externally peer-reviewed.
Concept - E.U., A.H.K.; Design - M.D., E.U., T.K.; Supervision - E.U., T.K., M.D.; Resource - E.U., M.D.; Materials - E.U., A.H.K., M.D.; Data Collection and/or Processing - E.U., M.K., M.D.; Analysis and/or Reviews - T.K., A.H.K., M.K.; M.D., E.U.
The authors have no conflicts of interest to declare.
The authors declared that this study has received no financial support.
We thank to Assistant Professor Dr. İbrahim İbiloğlu for his endeavors and also we thank to the Staff of Experimental Animal Center of Kahramanmaras Sutcu Imam University.