Ender Anılır1, Filiz Özen2, İbrahim Ali Özemir3, İbrahim Halil Yıldırım4, Çağrı Bilgiç5, Orhan Alimoğlu3

1Department of General Surgery, Amerikan Hospital, İstanbul, Turkey
2Department of Medical Genetics, İstanbul Medeniyet University School of Medicine, İstanbul, Turkey
3Department of General Surgery, İstanbul Medeniyet University School of Medicine, İstanbul, Turkey
4Department of Genetics, Dicle Üniversitesi School of Veterinary, Diyarbakır, Turkey
5Department of General Surgery, Koç University School of Medicine, İstanbul, Turkey


Objectives: There has been coverage of Toll-like receptor 4 and Toll-like receptor 2 gene polymorphisms in inflammatory episodes in a number of studies. In view of the inflammatory nature of acute pancreatitis, we aimed to determine the predictive value of mutations in Asp299Gly and Thr399Ile of the Toll-like receptor 4 gene, and the intron 2 microsatellite polymorphism of the Toll-like receptor 2 gene on the occurrence of acute biliary pancreatitis.
Materials and Methods: The study included 86 patients for the Toll-like receptor 4 Thr399Ile polymorphism, 100 patients for the Toll-like receptor 4 Asp299Gly polymorphism with acute biliary pancreatitis, and 101 healthy volunteers. At the same time, 93 patients and 92 healthy volunteers were included in the study to research the Toll-like receptor 2 intron 2 microsatellite polymorphism. Genotypes were determined using the restriction fragment length polymorphism analysis of PCR products and by an allele-specific PCR.
Results: The Toll-like receptor 4 Thr399Ile homozygotes mutant variants (p=0.005) and Toll-like receptor 2 MM genotype (p<0.001) were detected with a significantly higher frequency in patients with acute biliary pancreatitis than in the healthy blood donors.
Conclusions: The Toll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms and the Toll-like receptor 2 intron 2 microsatellite polymorphism are statistically associated with ABP.

Keywords: Acute pancreatitis, biliary, gene polymorphism, Toll-like receptor 2, Toll-like receptor 4

Cite this paper as: Anılır E, Özen F, Özemir İA, Yıldırım İH, Bilgiç Ç, Alimoğlu O. TLR4 Asp299Gly and Thr399Ile and TLR2 intron 2 microsatellite gene polymorphism in patients with acute biliary pancreatitis: Does it cause the disease?. Turk J Surg 2018; 10.5152/turkjsurg.2017.3828.

This study was presented at the “20.National Surgery Congress”, “13-17 April 2016”, “Antalya, Turkey”.


Ethics Committee Approval

Ethics committee approval was received for this study from the Ethics Committee of of İstanbul Medeniyet University (No. 2014-0064).

Peer Review

Externally peer-reviewed.

Author Contributions

Concept - E.A.; Design - E.A., İ.A.Ö.; Supervision - O.A.; Resource - E.A.; Materials - E.A., İ.H.Y.; Data Collection and/or Processing - E.A., C.B.; Analysis and/or Interpretation - F.Ö.; Literature Search - E.A.; Writing Manuscript -E.A.; Critical Reviews - İ.H.Y., F.Ö., İ.A.Ö.

Conflict of Interest

The authors have no conflicts of interest to declare.

Financial Disclosure

The authors declared that this study has received no financial support.