Investigating the effect of gold nanoparticles on hydatid cyst protoscolices under low-power green laser irradiation
Bayram Çolak1, Faruk Aksoy2, Selman Yavuz3, Mehmet Emin Demircili4
1Department of General Surgery, Selcuk University School of Medicine, Konya, Turkey
2Department of General Surgery, Necmettin Erbakan University School of Medicine, Konya, Turkey
3Department of Metallurgy and Materials Engineering, Selcuk University School of Engineering, Konya, Turkey
4Department of Medical Microbiyology, Necmettin Erbakan University School of Medicine, Konya, Turkey
Abstract
Objective: Various scolicidal agents are applied for the destruction of protoscolices in cysts media. Undesirable complications of the scolicidal agents limit the techniques to treat the cyst disease. Therefore, new non-toxic scolicidal agents are needed. Upon laser light irradiation, the photothermal gold nanoparticles (AuNPs) convert the absorbed laser light into heat through photothermal effect which kills the surrounding protoscolices by rising the temperature of the cysts media. In this study, we introduced biocompatible AuNPs as a non-toxic scolicidal agent to cure liver hydatid cysts.
Material and Methods: The protoscoleces were collected from the livers of naturally infected sheeps. In each experimental group, 1.5 mL suspensions of hydatid liquid containing protoscolices were added to test tubes. The test tubes were divided into five groups. Control, AuNPs only, Green laser only, High-dose AuNPs + laser and Low-dose AuNPs + laser groups. Two concentrations (0.4 and 0.8 mL) of AuNPs and three laser powers (30, 50, 150 mW) were applied for 30, 60 and 120 minutes to the groups. Then the ciysts liquid assessed under a light microscope and determined the viability of protoscoleces.
Results: Protoscolices in high-dose AuNPs group were destructed up to 89.30% deaths under 150 mW laser power for 120 minutes. However, negligible cell deaths were observed in cases where only AuNPs added or only laser irradiated groups. Increasing the dose of AuNPs or laser power or duration of aplication increased the protoscolosidal death rate.
Conclusion: In the study, we have successfully demonstrated that the AuNPs are an effective therapeutic and scolicidal agent to cure hydatid cyst disease under laser irradiation.
Keywords: Gold, nanoparticle, laser, hydatid cyst
Cite this article as: Çolak B, Aksoy F, Yavuz S, Demircili ME. Investigating the effect of gold nanoparticles on hydatid cyst protoscolices under low-power green laser irradiation. Turk J Surg 2019; 35 (4): 314-320.
Ethics approval for this study was granted by the ethical committee.
Written informed consent was obtained from patients who participated in this study.
Externally peer-reviewed.
Concept - B.Ç.; Design - B.Ç., F.A.; Supervision - F.A., S.Y.; Resource - B.Ç., M.E.D.; Materials - B.Ç.; Data Collection and/or Processing - F.A., B.Ç.; Analysis and/or Interpretation - B.Ç., M.E.D.; Literature Search - S.Y., B.Ç.; Writing Manuscript - B.Ç., F.A.; Critical Reviews - F.A.
The authors have no conflicts of interest to declare.
The authors declared that this study has received no financial support.