A novel coenzyme-Q approach for the prevention of postsurgical adhesion
Ramazan Kuşaslan1, Gülçin Ercan1, Orhan Ağcaoğlu2, Serdar Altınay3, Sinan Binboğa1, Yüksel Altınel1
1Department of General Surgery, Bagcilar Training and Research Hospital, Istanbul, Turkey
2Department of General Surgery, Koc University School of Medicine Hospital, Istanbul, Turkey
3Department of Pathology, Bagcilar Training and Research Hospital, Istanbul, Turkey
Objective: Postoperative intraperitoneal adhesions are an unsolved and important problem in abdominal surgery. In the present study, the probable preventive role of coenzyme-Q in the development of peritoneal adhesions was investigated.
Material and Methods: Sixteen Wistar Hannover male rats weighing 300-350 g were randomly separated into two groups of 8 rats each. The cecum was abraded with a sterile gauze until sub-serosal hemorrhage developed. A patch of peritoneum located opposite to the cecal abrasion was completely dissected. No treatment was given to Group 1. Group 2 received 30 mg/kg coenzyme-Q, which was injected 2 mL intraperitoneally. All the rats were sacrificed on the postoperative 21st day, and after adhesions were scored macroscopically, tissue specimens of the peritoneum and bowel were subjected to histopathological investigation. Tissue and blood specimens were also taken for biochemical analysis to investigate antioxidant efficiency.
Results: Adhesion scores were significantly different between the control group and the coenzyme-Q group (p= 0.001). According to the tissue levels of GSH-Px, MDA, and SOD levels, there was no significant difference between the study groups (p= 0.074, p= 0.208, p= 0.526). According to the plasma GSH-Px and SOD levels, there was significant difference between the groups (p= 0.002, p= 0.001), but the difference was not significant at MDA levels (p= 0.793). The differences between the pathological scores of the control and coenzyme-Q (p= 0.028 for fibrosis; p= 0.025 for inflammation) groups were statistically significant.
Conclusion: This study confirms that coenzyme-Q is the potential application in the prevention of early postoperative adhesions.
Keywords: Coenzyme Q, adhesion, surgery
Cite this article as: Kuşaslan R, Ercan G, Ağcaoğlu O, Altınay S, Binboğa S, Altınel Y. A novel coenzyme-Q approach for the prevention of postsurgical adhesion. Turk J Surg 2020; 36 (2): 202-208.
Consent was received from the Bağcılar Education and Research Hospital Experimental Animals Production and Research Laboratory Ethical Committee (HADYEK/2013-02/28.01.2013).
Informed consent form was obtained from all patients.
Concept – R.K.; Design – R.K., S.A.; Supervision – R.K.; Resource – S.A., O.A.; Materials – G.E., S.A.; Data Collection and/or Pro-cessing – S.B., O.A.; Analysis and Interpretation – O.A., G.E.; Literature Review – S.B., Y.A., G.E.; Writing Manuscript – R.K., S.B. Y.A.; Critical Reviews – Y.A.
The authors have no conflicts of interest.
Financial support was provided by departmental sources.