Association of CDH1 -160 C → A and -347 G→ GA polymorphisms and expression of E-cadherin and gastric cancer: A case-control study
Adem Akçakaya1, Nurcan Ünver2, Tuğba Aydoğan Kiriş3, Mehmet Güzel4, Fatma Betül Akçakaya5, Bedia Çakmakoğlu6, Mustafa Hasbahçeci7,8
1Department of General Surgery, Bezmialem Vakif University, Faculty of Medicine, İstanbul, Turkey
2Clinic of Pathology, Yedikule Chest Diseases and Thoracic Surgery Training and Research Hospital, İstanbul, Turkey
3Graduate School of Science, İstanbul University, İstanbul, Turkey
4Clinic of General Surgery, Patnos State Hospital, Ağrı, Turkey
5Bezmialem Vakif University, Faculty of Medicine, İstanbul, Turkey
6Department of Molecular Medicine, Istanbul University Aziz Sancar Institute of Experimental Medicine, İstanbul, Turkey
7Department of General Surgery, Istanbul Istinye University, Faculty of Medicine, İstanbul, Turkey
8A.D.E.M., Center for Academic Support and Education, Istanbul, Turkey
Abstract
Objective: The loss of function of the E-cadherin (CDH1) gene with -160 C→A and -347 G→GA polymorphisms is regarded as a critical step for gastric cancer. It was aimed to investigate possible association of these polymorphisms and immunoexpression of E-cadherin with gastric cancer.
Material and Methods: Gastric adenocarcinoma patients and individuals with benign gastric pathologies were included in this case-control study. Demographic data and pathological findings were recorded. Immunohistochemical staining of E-cadherin expression and analysis of -160 C→A and -347 G→GA polymorphisms were done. Differences between allele frequencies of -160 C→A and -347 G→GA polymorphisms and expression of E-cadherin were the primary outcomes.
Results: There were 78 gastric cancer patients (Group A) and 113 individuals with benign gastric pathologies (Group B). The number of male patients and mean age were higher in Group A (p< 0.001). -160 C→A and 347 G→GA polymorphisms and their allelic distributions showed no difference between the groups (p> 0.05 for all). There was a significant association between -160 C→A polymorphism and grade of E-cadherin expression (p= 0.013). There were no significant differences between survival rates with -160 C→A, 347 G→GA and intensity of E-cadherin expression (p> 0.05 for all). There was no significant association between -160 C→A and -347 G→GA polymorphisms and gastric cancer.
Conclusion: There was no impact of E-cadherin expression on tumoral features and survival in gastric cancer. -160 C→A polymorphism may influence the expression of E-cadherin in gastric cancer.
Keywords: CDH1 polymorphisms, E-cadherin, gastric carcinoma, immunohistochemical expression, survival
Cite this article as: Akçakaya A, Ünver N, Aydoğan Kiriş T, Güzel M, Akçakaya FB, Çakmakoğlu B, et al. Association of CDH1 -160 C → A and -347 G→ GA polymorphisms and expression of E-cadherin and gastric cancer: A case-control study. Turk J Surg 2021; 37 (1): 39-46.
The approval for this study was obtained from Bezmialem Vakif University Clinical Research ethics Committee (Decision no: 71306642/050-01-04/296 Date: 22.10.2014).
Externally peer-reviewed.
Concept - M.H., A.A., B.Ç., N.Ü.; Design - M.H., M.G., A.A.; Supervision - M.H., A.A., B.Ç.; Materials - M.H., A.A., M.G., N.Ü., B.Ç.; Data Collection and/or Processing - All of authors; Analysis and Interpretation - M.H., N.U., B.C.; Literature Review - M.H., F.B.A., M.G.; Writing Manuscript - M.H., T.A.K., F.B.A., M.G.; Critical Reviews - M.H., A.A., B.C.
The authors declare that they have no conflict of interest.
The authors declared that this study has received no financial support.
Authors were thankful to Ms. Kadriye Gumus for her kind support for English editing.