Does taurolidine have any effect on liver regeneration and oxidation in the experimental hepatectomy model?
Emrah Akın1, Kaya Sarıbeyoğlu1, Eren Esen1, Erman Aytaç1, Gülşen Özbay2, Hafize Uzun3, Salih Pekmezci1
1Department of General Surgery, Istanbul University Cerrahpaşa Faculty of Medicine, Istanbul, Turkey
2Department of Pathology, Istanbul University Cerrahpaşa Faculty of Medicine, Istanbul, Turkey
3Department of Biochemistry, Istanbul University Cerrahpaşa Faculty of Medicine, Istanbul, Turkey
Abstract
Objective: Taurolidine is a bicyclic molecule produced by the natural amino acid taurine. Antibacterial, antiendotoxic and cytoprotective effects of taurolidine have been shown experimentally. Data on the effects of taurolidine on oxidative stress and hepatic regeneration are limited. The aim of the study was to evaluate the effect of taurolidine on hepatic regeneration and oxidative stress in rats undergoing partial hepatectomy.
Material and Methods: Forty adult, male Wistar Albino rats were randomly divided into four equal groups: sham (S) group (n= 10), post-sham opera- tion taurolidine administered (ST) group (n= 10), partial hepatectomy (H) group (n= 10) and post-partial hepatectomy taurolidine administered (HT) group (n= 10). 100 mg/kg/day taurolidine was administered for seven days. Blood and liver tissue samples were collected on postoperative day seven. Liver tissue malondialdehyde, glutathione and Cu-Zn superoxide dismutase activity (SOD) were measured to assess oxidative stress. Binuclear hepato- cyte and Ki-67 antigen levels were measured to evaluate hepatic regeneration.
Results: There was no difference between the groups for malondialdehyde, Cu-Zn superoxide dismutase and glutathione levels (p> 0.05). Binuclear nuclei levels were comparable between the H and HT groups (p= 0.06), while taurolidine decreased binuclear hepatocyte levels in the sham operated groups (p= 0.02). Taurolidine application decreased Ki-67 levels after partial hepatectomy (p= 0.001).
Conclusion: Taurolidine may cause anti-regenerative effects after partial hepatectomy without causing oxidative damage.
Keywords: Oxidative stress, liver resection, hepatic regeneration, partial hepatectomy
The approval for this study was obtained from Istanbul University Animal Experiments Local Ethics Committee (Decision No: 41, Date: 16.04.2009).
Externally peer-reviewed.
Concept - E.A., K.S., E.A.; Design - E.A, K.S., E.A.; Materials - E.A., E.E.; Data Collection and/or Processing - E.A., G.Ö., H.U.; Literature Review - E.A.; Writing Manuscript - E.A., K.S., E.A.; Critical Reviews - K.S., S.P.
The authors declare that they have no conflict of interest.
The authors declared that this study has received no financial support.