THE EFFECT OF L-GLUTAMINE ON INTESTINAL ADAPTATION IN THE RATS AFTER SIALOADENECTOMY AND SMALL BOWEL RESECTION

Abstract

Luminal foods, endogenous secretions and hormones are effective in the process of intestinal adaptation after intestinal resection. Submandibulary gland is the main source or EGF(Epidermal Growth Factor) in rats. Removal of the glands(Sialoadenectomy) will cause depletion of EGF in rats. The aim of this study was to investigate the effect of L-glutamine to compensate the effect of sialoadenectomy during massive intestinal resection in rats. This study was performed in the Medical Research Center Of xx. Forty five rats were fed by mouth with TPN solution before 48 hours and for seven days after the operation. The rats were divided into 3 groups each of which included 15 rats. Group 1: Sham sialoadenectomy + small bowel resection + oral TPN solution. Group II: Sialoadenectomy + small bowel resection + oral TPN solution. Group III: Sialoadenectomy + small bowel resection + oral TPN solution + L-Glutamine. All rats were sacrificed at 7th day of the operation for the evaluation of intestinal adaptation. There was a significant increase in villous length and crypt depth, in group III when compared to Group I and II (p=0.0001). There was also a meaningful decrease in villous length and crypt depth in Group II compared to Group I (p=0.0001 ). There was no significant changes in goblet cell proliferation between the groups(p>0.05). L-glutamine can prevent mucosal atrophy like EGF in rats with sialoadenectomy and small bowel resection.