Şeyma Özsoy1, Zeki Özsoy2, Fikret Gevrek3, Abdullah Özgür Yeniova4

1Department of Physiology, Tokat Gaziosmanpaşa University Faculty of Medicine, Tokat, Türkiye
2Department of General Surgery, Tokat Gaziosmanpaşa University Faculty of Medicine, Tokat, Türkiye
3Department of Histology, Tokat Gaziosmanpaşa University Faculty of Medicine, Tokat, Türkiye
4Department of Internal Medicine and Gastroenterology, Tokat Gaziosmanpaşa University Faculty of Medicine, Tokat, Türkiye


Objective: Inflammatory bowel disease (IBD) is a chronic, relapsing, and remittent inflammatory disease of the gastrointestinal tract. Nutritional deficiency may be instrumental in and attributable to this disease. We examined the effect of VitB12 supplementation on acetic acid (AA)-induced colitis in rats.

Material and Methods: Five minutes after the application of acetic acid to the rats to create a colitis model, VitB12 was administered 1 mg/kg, i.p concentration, then the application continued for three consecutive days. Control groups were included for colitis and VitB12. After 4d, the rats were sacrificed, and colonic tissues were harvested for macroscopic and microscopic examination of colonic damage. TNF-α, IL-1β, IL-6, MDA, GSH and SOD values were measured biochemically.

Results: There was statistically significant macroscopic improvement in damage to the colon tissues (p< 0.05). The severity of inflammation reduced in the VitB12 treated rat group compared with the control group, but was not significantly. The levels of TNF-α, IL-1β, MDA, and SOD did not differ between AA control and VitB12 treated AA colitis group. However, the levels of IL-6 and GSH were statistically significant different in rats with AA-induced colitis after VitB12 injection (p< 0.05).

Conclusion: Nutritional deficiencies might contribute to the pathogenesis of IBD, and the efficacy of VitB12 supplementation has controversial effects on the intestinal mucosa.

Keywords: Vitamin B12, inflammatory bowel disease, inflammation, acetic acid

Cite this article as: Özsoy Ş, Özsoy Z, Gevrek F, Yeniova AÖ. Protective role of vitamin B12 on acetic acid induced colitis in rats. Turk J Surg 2023; 39 (1): 7-16.


Ethics Committee Approval

This study was approved by Gaziosmanpaşa University Rectorate Animal Experimentation Local Ethics Committee (Decision no: 51879863-183, Date: 08.05.2014).

Peer Review

Externally peer-reviewed.

Author Contributions

Concept – SO, ZO; Design - SO, ZO; Supervision - SO, ZO, FG; Funding – ZO; Materials - SO, ZO, FG; Data Collection and/or Processing - SO, ZO, FG; Analysis and/or Interpretation - SO, ZO, FG, AOY; Literature Review - SO, ZO, FG, AOY; Writer- SO, AOY; Critical Review - SO, ZO, AOY.

Conflict of Interest

The authors have no conflicts of interest to declare.

Financial Disclosure

The authors declared that this study has received no financial support.


We are greatly indebted to professor Ki Baik Hahm for his help and critical suggestions during preparing the final version of manuscript.