THE EFFECTS OF CHOLECYSTOKININ ANTAGONIST IN EXPERIMENTAL ACUTE PANCREATITIS

Abstract

Aims: The aim of this experimental study was to evaluate effects of cholecystokinin receptor antagonist Lorglumide in an experimental model of acute pancreatitis in rats caused by closed duodenal loop.

Background: Several studies suggest that cholecystokinin which is an endocrine hormon plays apermissive or contributory role in acute pancreatitis. Since 1979, several competitive receptor antagonists of the cholecystokinin have been developed and therapeutic potential effects of these agents had been investigated in experimental acute pancreatitis models.

Material and Methods: After creation of closed duodenal loop 30 rats were divided into two groups, then the CCK-antagonist Lorglumide at 10 mg/kg or 0.9 % NaCl were given intraperitoneally every 6hours. Five rats in each group were sacrified 6,12,24 hours after the closed duodenal loop creation. Following sacrification serum and pancretic tissue were taken for biochemical and morphological studies and wet weight of pancreas was measured.

Results: The increase in serum amylase and lipase concentration after closed duodenal loop creation was significantly reduced by Lorglumide at all time intervals when compared with the NaCl group (p<0.05) Lorglumide ameliorated ruptures of ducts, hemorrhage and fat necrosis (p<0.05). Pancreatic wet weight was not effected by cholecystokinin antagonist (p>0.05).

Conclusion: Closed duodenal loop creation causes acute pancreatitis in rats. Cholecystokinin antagonist had beneficial effects in this experimental acute pancreatitis model but is not able to eradicate the pancreatitis in this setting.