THE EFFECTS OF ESTRADIOE, ESTRONE, ESTRIOL AND PROGESTERONE ON RAT MAMMARY GLANDS

Abstract

The aim of the study was to determine the histopathological changes and to evaluate the results when the estrogens and progesterone hormones were enjected intraparanchimaly on the rat mammary gland in an experimental animal model. The lobuloalveolary progress was demonstrated by the enjection of estrogen to the areola of mammary gland of donkey, rabbit and pig, invivo studies. Fifty Sprague-Dowley female rats weighing 200 to 250 grams were divided into five groups. Estradiol (5 mg/kg), estriol (0.5mg/kg), estrone (0.5 mg/kg), progesterone (0.6 mg/kg) and serum phisyology (2 cc/kg) were enjected intramammary. The blood samples were taken prior to the sacrification of animals for tumor markers, CEA and Ca 15.3 and simple mastectomy was performed. In the study, higher rates of mitosis, increasing in cell sequence and pleomorphism was observed especially in estradiol and estrone groups. Serum CEA and Ca 15.3 levels were also higher in the same group of animals, Mitosis, increasing in cell sequence and pleomorphism were statistically significant in estradiol and estrone groups (p<0.05). Serum CEA and Ca 15.3 levels were also found to be higher in the same groups (p<0.05). We consider that estrogens especially estrone and estradiol when enjected intramammary may cause histopathologic changes which so called precancerogenic in ductal cells of rats in time.