EFFECT OF MIDEZOLAM ON ATTENUATION OF INTRAPERITONEAL HYPERTHERMIC STRESS IN RATS

Abstract

In thisstudy,the effect of 41 -43 C hyperthermy used for intraperitoneal hyperthermic chemoperfusion treatment protocol on stress response and the effect of midazolam to this response was investigated. For this study, 18 wistar rats were divided into three groups. All groups were performed laparotomy following the sampling of 0th hour blood and four nelaton drains were inserted into the abdominal cavity namely two into the upper and two into the lower half before the closure of the abdomen. Group 2 (Control): 100ml of normothermic lactated Ringer's solution was administered through the upper drain and taken back through the lower ones.

Group 2: By using the same procedure lactated Ringer that was heated up to 43 C was perfused. Group 3: 5mg/kg midazolam was injected i.m. route just before the hyperthermic perfusion.

After one hour perfusion blood samples were taken from all subjects for insulin and cortisol levels. Cortisol levels were different at the 0th and end of the experiment significantly for all three groups. As the insulin levels were compared, it was found to be suppressed significantly in the groups 2 and 3. For the1ist hour cortisol levels; levels of the group 2 was higher significantly. For the end of the experiment insulin levels it was seen that all three groups were different and most significant insulin suppression was in group 1.

As a result midazolam a benzodiazeine has a reducing effect on stress response arised due to hyperthermia.