TROPHIC EFFECTS OF RECOMBINANT HUMAN GROWTH HORMONE ZINC SULPHATE, AND L-GLUTAMINE ON DEFUNCTIONED SMALL BOWEL IN RATS

Abstract

The aim of this study is to investigate the effect of two nutrients and one hormone on defunctioned intestinal segment in rats. A vascularized jejunal loop of 15 cm in length was prepared and separated from the intestine and its two ends brought out through the abdominal wall as mucous fistulas, intestinal continuity was restored by jejunojejunostomy. The rats were divided in to five groups, each of which had 10 animals. All groups had standard nutrition by oral route. First group (control) received only oral parenteral nutrition (TPN) solution, in addition to oral TPN solution the second group had recombinant human growth hormone (0.4 mg/kg/day), 3rd group ZnSO4 (1.0 mg/kg/day), the 4th group L- glutamine (0.2 g/kg/day) and the 5th group combination of last three additives with the same dosages. At the end of the third week, all rats underwent laparatomy. The defunctioned and functioned segments were resected for mucin (Goblet cells were counted) and length of villi at light microscopic level to evaluate histological criterias. For the examinated two parameters all experiment groups showed statistical meaningful results.

In conclusion it is observed that the mentioned hormone and nutrients have trophic effects on rat defunctioned intestinal segment. They also have a protective effect for intestinal atrophy. The best results were obtained in the combined group.