THE EVALUATION OF BACTERIAL TRANSLOCATION IN ACETIC ACID INDUCED EXPERIMENTAL COLITIS ON RATS

Abstract

Due to the mucosal injury the change of luminal bacterial flora and spread of the enteric bacteria to other organs by passing through the mucosa are expected to occur in the inflammatory bowel disease. By the purpose of evaluating the above mentioned case, the two groups of rats were examined.

The control group (no: 8), which was rectally irrigated by 9% saline solution and the coin group (n: 10), by 5% acetic acid were formed. Both of these groups were examined clinically and histopathologically with respect to presence of colonic inflamation. Bacterial count of colonic flora and bacterial translocation were evaluated. There was a significant loss of body weight of rats in the colit group (t:5 81, p<0.001) while there was none in the control group. The ratio of colonic segment weight to the total body weight was found significantly higher in the coin group than in the control group (t:5.06 p<0.00001). The colonic macroscopic inflamation score was higher in the colit group when compared with that of the control grup (u:80, p<0.05 mwu test). When the bacterial examination of the luminal faeces was done using log10 (c.f.u per gram), aerobic gram (-) bacilles (t: 2, 68, p<0.02) and anaerobic gram (-) bacteria (t:2.49, p<0.03) were signigficantly high in the colit group in comparison with those in the control group. Whereas anaerobic gram (+) bacteria were significantly low (t: 2, 45 p<0.03) in comparison with those in the control group.

When the bacterial translocation to the organs was observed the presence of bacteria in the following organs were found to be 90% in mesenter lymph nodes, 80% in liver, 40% in lung, 30% in systemic blood and 10% in spleen. E.coli and anaerobic gram (-) bacteria were determined to have cultured most in the organs. There was positive correlation between the colonic inflamation score and bacterial translocation to the organs.