THE EFFECTS OF FIBROUS RICH FOODS AND ANTIBIOTICS IN TOTAL PARENTERAL NUTRITION FOR THE BACTERIAL TRANSLOCATION

Abstract

The relationship of the feeding regimen and the bacterial translocation which has been accepted as a source of sepsis is still being investigated. Seven groups of Wistar-Albino rats were used in this experimental study. Total parenteral nutrition was administered to two groups orally and intravenously for one week. Cellulose (2.5 g/day) and Clindamycine (10 mg/kg/day) were added to the protocolen in four groups. At the end of the seven days, all rats were killed and cultures were taken from the mesenteric lymph nodes and colonic intraluminal fluid. Biopsies were taken from terminal ileum for histopathological examination and the results were compared with the control group. Bacterial translocation at the mesenteric node was found 50-62.5% in oral and intravenous total parenteral nutrition groups, it was seen the mucosal disruption with intraluminal over-colonization of the bacteria. Mesenteric lymph node translocation in feeding with cellulose group was found 0-70% and mucosal integrity was protected and there was no difference in the intraluminal colonization of bacteria between the control groups. The mesenteric translocation was found 0-20% in oral total parenteral nutrition which is combined with antibiotic therapy and the mucosal integrity of intestine was not protected but intraluminal colonization of bacteria was found lesser than the control group. In this study, we showed that intraluminal colonization of bacteria is important as the mucosal integrity of intestine in bacterial translocation.