THE EFFECT OF CYCLOSPORINE A ON EXOCRINE PANCREATIC FUNCTION OF PANCREATECTOMISED RATS
YUSUF ERÇİN SÖNMEZ, ŞÜKRÜ DİLEGE, NEJAT SAVCI, SÜHENDAN EKMEKÇİOĞLU, OSMAN ÖZTÜRK, SEVİM BÜYÜKDEVRİM
İstanbul Üniversitesi, İstanbul Tıp Fakültesi, DETAM (Deneysel Tıp Araştırma Merkezi)/İSTANBUL
Abstract
Ninty male Wistar rats weighing 200-240gr each were used throughout the study. The effect of 5mg/kg/ and 10mg/kg CyA on exocrine pancreatic function of rats which were pancreatectomised 50 and 80% was studied. The results obtained were compared with the results of the rats which were not pancreatectomised, but which were fed or not fed with the same amounts of CyA.
Cyclosporine A was given subcutaneously during 10 days. On the 10th day blood CyA levels were measured using RIA method were 158±12.7ng/ml and 285±14.3ng/ml obtained from the rats fed with 5mgand 10mg/kg/day CyA successively.
On the 10th day, after the CCK-8 stimulation these results were obtained: Amylase and lipase levels were impaired in rats administered with 10mg/kg CyA and in rats which were submitted to a 50 or 80% distal pancreatectomy and administered 5mg/kg/day CyA. Blood trypsin values were decreased significantly in the rats administered 10mg/kg/day CyA and which were submitted to a 50 or 80% distal pancreatectomy.
Light microscopic histopathological evaluations of the pancreatic tissue samples were performed on the10th day and the most noxious effects of CyA were noted in the group which was given 10mg/kg/day of CyA.