Dr. Orhan ÇELEN, Dr. Tahsin DALGIÇ, Dr. Uğur BERBEROĞLU

Ankara Onkoloji Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, ANKARA


In this study, the effect of granulocyte colony stimulating factor (G-CSF) on tumor necrosis factor alpha (TNF-α) and prognosis in an experimental model of peritonitis caused by cecal ligation and puncture were evaluated. The rats were divided into three groups. Cecal ligation and puncture (CLP) was performed to the first group, CLP and granulocyte colony stimulating factor (G-CSF) to the second group, simple laparotomy to the third group. The pre- and postoperative TNF-α levels were obtained and rats were observed every six hours for survival analysis. The postoperative TNF-α levels were found to be elevated in CLP group and administration of G-CSF reduced the elevated TNF-α levels. The survival rates were significantly better in the CLP+G-CSF group. The study has shown that administration of G-CSF reduced the elevated TNF-α levels due to peritonitis and prolonged survival.

Keywords: Tumor necrosis factor alpha (TNF-α), granulocyte colony stimulating factor (G-CSF), sepsis, peritonitis.