THE EFFECT OF EXTERNAL/ INTERNAL DRAINAGE OR ORAL ADMINISTRATION OF BILE COMPONENTS ON HEPATIC REGENERATION IN OBSTRUCTIVE JAUNDICED RATS WITH PARTIAL HEPATECTOMY

Abstract

In the present study, the effects of internal and external drainage and oral administration of bile components on hepatic regeneration of obstructive jaundiced rats with hepatectomy were studied by evaluating the immunohistochemical criteria. Common bile ducts of rats were cannulated and the catheter was bent and ligated to produce obstructive jaundice. On the third day of the obstruction, rats underwent 50 % hepatectomy. Then, the ligated catheters of the rats were surgically inserted into the duodenum for internal drainage (ID) or inserted into the bladder for external drainage (ED). Rats in the ED groups were orally given saline (S), cholesterol-lecithin (CL) or bile salt (BS) for 3 or 7 days. On the 3^rd or 7^th day of the treatments, rats were decapitated, blood samples were taken for the analysis of hepatic function tests, liver wet weights were recorded and hepatic samples were stored to assess PCNA and Ki-67 by immunohistochemical methods. On the third day, the hepatic regenaration rates with PCNA method in the CL group were significantly higher when compared to saline-treated external drainge and internal drainage groups. On the seventh day, the hepatic regeneration rates were significantly lower compared to the bile duct ligation group. On the third day, the hepatic regenaration rates determined by Ki-67 method were significantly higher in the CL group. On the seventh day, the hepatic regeneration rates of the S-treated ED group were found to be significantly lower compared to the internal drainage group and the BS-treated ED group. In conclusion, external drainage group which received only saline demonstrated lower hepatic regeneration compared to internal drainage group in both the early and the late periods. It is found that when CL and BS are given in the external drainage groups, especially in the early period, regeneration rates reach higher values and become even higher than that observed in the internal drainage group. However in the late period, CL or BS administrations in the external drainage groups approximate the regeneration rates to those of internal drainage group.