THE EFFECTS OF THE N-BUTYRATE AND L-GLUTAMIN ENEMAS ON THE COLONIC MUCOSA AND PORTAL ENDOTOXEMIA IN EXPERIMENTAL COLITIS
Dr. Ekrem KAYA1, Dr. Pınar TUNCEL2 Dr. Halil ÖZGÜÇ3, Dr. Rıfat TOKYAY 3
1Ondokuz Mayıs Üniversitesi Tıp Fakültesi Genel Cerrahi ABD, SAMSUN
2 Dokuz Eylül Üniversitesi Tıp Fakültesi, Biyokimya ABD, İZMİR
3Uludağ Üniversitesi Tıp Fakültesi, ilk ve Acil Yardım ABD, BURSA
Abstract
The aim of this study was to investigate the effects of the n-butyrate and l-glutamine enemas on mucosal inflammation and portal endotoxemia in experimental induced colitis. The etiology of the inflammatory bowel disease (IBD) is still unknown. Efforts In trial to describing the pathogenesis of IBD has recently increased. Some theraupetlc modalities were developed during this experimental and clinical studies. Colitis was induced with 5% asetic acid enema in rats. Saline (n: 10), n-butyrate (n: 12), l-glutamine (n: 12) enemas were applied twice daily to the rats for seven days after induction of colitis, Sham group (n: 12) received only saline enema without induction of colitis. Rats were sacrified at the ences between colitis and treatment groups were insignificant. The level of colonic mucosal GSH and portal endotoxin of all groups were not statistically different . n-Butyrate enema reduces colonic inflammation macroscopically. As a result of this study, portal endotoxemia and colonic mucosal GSH level measurements are not reliable indicator in assessing the severity of inflammation in experimental induced colitis.
Keywords: Experimental colitis, butyrate, glutamine, enema, mucosal glutathione, portal endotoxin